Aims: A simple and rapid method was described for DNA isolation directly from activated sludge or other environmental sources, including soil and sediments. Methods and Results: The present method is based on microwave thermal shock and provides DNA suitable for further analysis. It is also effective for RNA extraction. Conclusions: The protocol is effective, easy, fast and does not require the use of expensive equipment or reagents. Significance and Impact of the Study: The described method can be applied to difficult substrates in environmental microbiology studies.
A microwave-based method for nucleic acid isolation from environmental samples
Romano Spica V
2001-01-01
Abstract
Aims: A simple and rapid method was described for DNA isolation directly from activated sludge or other environmental sources, including soil and sediments. Methods and Results: The present method is based on microwave thermal shock and provides DNA suitable for further analysis. It is also effective for RNA extraction. Conclusions: The protocol is effective, easy, fast and does not require the use of expensive equipment or reagents. Significance and Impact of the Study: The described method can be applied to difficult substrates in environmental microbiology studies.File in questo prodotto:
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